Pipeline Pool


To install and run a pipeline, please clone its repository from the pipelines/ directory of the PluMA source tree. Have a new pipeline that you would like to add to the pool? Please send your repository and a description to Trevor Cickovski.
Completed, Released
These bioinformatics pipelines have been released in current publications.
ABR Antibiotic Resistance (Stebliankin et al, 2022)
ADHD ADHD 16S gut microbiome analysis (Cickovski et al, 2023)
Alpha1 Alpha-1 Antitrypsin Deficiency multiomics analysis (Cickovski et al, 2019)
Alpine Transcript abundance estimation by modeling RNAseq fragment bias (Love et al, 2016)
AmpliconNoise Remove noise from 454 amplicons (Quince et al, 2011)
AmpliQue Bacterial identification using 16S primer data (Gonzalez et al, 2008)
AMR Antimicrobial resistance (Steblinakin et al, 2022)
Antibiotics Influence of antibiotics on bacterial growth (Stebliankin et al, 2021)
ASV Convert raw metagenomics data to Amplicon Sequence Variants (ASVs) (Callahan et al, 2017)
BacterialGrowth Bacterial growth using peak-to-trough ratio (Stebliankin et al, 2020)
Bayes Bayesian network (Auguet et al, 2021)
BaySeq Empirical Bayesian methods for identifying differential expression (Hardcastle et al, 2010)
BEDTools BEDTools (Quinlan et al, 2010)
Benchmark Challenges in benchmarking metagenomic profilers (Sun et al, 2021)
BRENDA Multiomics analysis using the BRENDA (Casero-Cheema et al, 2017) enzyme database
Cache Machine learning and cache block replacement (Yusuf et al, 2021)
Cancer Pangenome cancer genome analysis (Poore et al, 2020)
CaReT Classification And REgression Training (Kuhn et al, 2019)
Causality Compute causal networks from microbiome abundance data (Sazal et al, 2019)
COVID19 Molecular mimicry with Covid-19 Spike protein (Nunez-Castilla et al, 2022)
CQN Conditional Quantile Normalization Method for RNA-Seq (Hansen et al, 2012)
CysticFibrosis 16SrRNA variable regions, cystic fibrosis study (Doud et al, 2010)
DBN Build a Dynamic Bayesian Network from longitudinal microbiome data (Ruiz-Perez et al, 2019)
DEGSeq Identify Differentially Expressed Genes from RNASeq data (Wang et al, 2010)
Derfinder Annotation-agnostic differential expression analysis of RNA-seq data at base-pair resolution (Collado-Torres et al, 2017)
DRIMSeq Differential transcript usage and tuQTL analyses with Dirichlet-multinomial model in RNA-seq (Nowicka and Robinson, 2016)
EBSeq Empirical Bayes Differential Expression analysis at both gene and isoform level using RNA-seq data (Ma et al, 2024)
Electricity Apply transformers to predict electricity consumption (Li et al 2020, Sama 2020)
Environment Rubin causal model for estimating environmental exposure effects on microbiome (Sommer et al, 2021)
Epitopedia Identifying molecular mimicry between pathogens and known immune epitopes (Balbin et al, 2022)
Flint Large-scale microbiome analysis in the cloud (Valdes et al, 2019)
Flood Flood prediction using machine learning (Shi et al, 2022)
Glutamine Analysis of glutamine metabolite (Cickovski et al, 2021)
GOExpress Visualise microarray and RNAseq data using gene ontology annotations (Rue-Albrecht, 2024)
GPU Miscellaneous GPU-based pipelines using NVIDIA Kits (NVIDIA, 2016)
GulfWar Mouse Gulf War Illness study (Seth et al, 2022)
HADDOCK High Ambiguity Driven protein-protein DOCKing (Honorato et al, 2024)
HELIUS Analysis of the HELIUS dataset (Nayman et al, 2023)
HIVEvoNets Evolutionary networks of sequence data and application to HIV (Buendia and Narasimhan, 2006)
Immunotherapy Impact of immunotherapy on cancer microbiome (Stebliankin et al, 2019)
InfantGut Infant gut microbiome analysis (Ruiz-Perez et al, 2019)
Jasper Microbiome map assembly (Valdes et al, 2021)
Kaiju Kaiju taxonomic classification (Menzel et al, 2016)
Kidney Kidney microbiome analysis (Park et al, 2020)
LSC Improving PacBio Long Read Accuracy by Short Read Alignment (Au et al, 2012)
MASH Fast genome and metagenome distance estimation (Ondov et al, 2016)
MathTest Starter pipeline for PluMA, that performs basic arithmetic (Vargas, 2022)
MeRRCI Metagenome, Resistome, Replicome for Causal Inferencing (Stebliankin et al, 2022)
Metabolomics Metabolomics pipeline (Stebliankin et al, 2022)
Metronidazole Effect of metronidazole on vaginal microbiota (Ruiz-Perez et al, 2021)
MOSAIC MOSAIC pipeline to compute Index of Replication (Stebliankin et al, 2020)
Mouse Metagenomics pipeline to analyze 16S mouse gut microbiome data, from the PluMA userguide (Kozich et al, 2013)
MSN Produce microbial social networks (Fernandez et al, 2015)
OLego OLego DeNovo mRNA splicing (Zhang et al, 2023)
Opioid Microbiome-related relationships between cocaine use and metabolites (Martinez et al, 2022)
OriginOfReplication Compute the origin of replication (Brown et al, 2016)
OTU_ASV OTU-ASV comparison on 16S cohort gut microbiome dataset (Nayman et al, 2022)
PALM Pipeline for the Analysis of Longitudinal Multi-omics time-series data (Ruiz-Perez et al, 2021)
PanACEA Pan-genome Atlas with Chromosome Explorer and Analyzer (Clarke et al, 2018)
Parkinsons 16S gut microbiome dysbiosis in Parkinson's Disease patients (Pietrucci et al, 2019)
PetRI PetRi pipeline for determining bacterial replication rates (Stebliankin et al, 2020)
PIsToN Protein binding Interfaces with Transformer Networks (Stebliankin et al, 2023)
Plots Split a dataset, run compositional and differential analyses, compute correlations, produce plots (Aguilar-Pulido et al, 2015)
PLSDASoYouThink Partial Last Square Discriminant Analysis (PLSDA) Performance (Ruiz-Perez et al, 2020)
PM16S Downstream 16S mouse gut microbiome analysis, pre- and post-obesity (Cickovski and Narasimhan, 2018)
PRADA Prioritization of Regulatory Pathways based on Analysis of RNA Dynamics Alterations (Torres-Garcia et al, 2014)
PRIMAL Pipeline for Retrieval of Interactions in the Microbiome from temporAL data (Lugo-Martinez et al, 2019)
ProcessAbund Take raw abundances, normalize and threshold them, and split into groups (Riveros et al, 2014)
Qiime2 Qiime2 metagenomics pipeline (Polanco et al, 2020)
RAPToR Resistome Abundance and Peak-to-Trough Ratio Pipeline (Stebliankin et al, 2022)
RedTide Gut microbiome analysis of red-tide microcystin exposed mice (Saha et al, 2022)
REPTILE Regulatory DNA Element Prediction (He et al, 2016)
RNASeqComp Benchmarks for RNA-seq Quantification Pipelines (Teng et al, 2016)
RNASeqReadSimulator Produce synthetic RNA sequences with mutations (Li et al, 2013)
SAHMI Denoising sparse microbial signals from single-cell sequencing of mammalian host tissues (Ghaddar et al, 2022)
SGSeq Prediction and Quantification of Splice Events from RNA-Seq Data (Goldstein et al, 2014)
Smoking Smoker 16S lung microbiome study (Campos et al, 2022)
Tara Structure and function of the global ocean microbiome (Sunagawa et al, 2015)
T2D Type-2 Diabetes Mellitus (Nayman et al, 2024)
Tensorflow Pipeline with miscellaneous Tensorflow utilities (Rodriguez and Riggs, 2016)
Tuxedo Differential gene and transcript expression analysis (Trapnell et al, 2012)
Viral Viral challenge (2016)
VPhyloMM Viral mutational pathways of drug resistance (Buendia et al, 2009)


Completed, Not Released
These bioinformatics pipelines will be released in future publications.
EMoMiS Epitope-based molecular mimicry search pipeline (Stebliankin et al, 2023) Submitted
IBD Irritable Bowel Disorder (IBD) cohort analysis (Schreiner et al, 2024)
PCOS Poly-Cystic Ovary Syndrome gut microbiome study (Menendez et al, 2024)
Pseudomonas Pseudomonas genome evolution (Rodriguez et al, 2022)
Soil Impact of heavy metal exposure on soil microbiome (Morales et al, 2024)
UMiami16S 16S pipeline, (Exercise, Duodenal Adenoma, Microbiota/Macrophage) with UMiami-FL (Jacobsen et al, 2024) Submitted
Veg3CAMR Antimicrobial resistance using 3C denovo assembly (Sakthivel et al, 2024) Submitted


In Progress
These bioinformatics pipelines are currently being constructed.
RNASeq Microbiome and host RNAseq pipeline (Jacobsen et al, 2024)
SingleCell Single-cell RNA network analysis (Mendygral et al, 2025)